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National Mission on Himalayan Studies

Implemented by the Ministry of Environment, Forest & Climate Change (MoEF&CC)
Nodal and Serving hub with G.B. Pant National Institute of Himalayan Environment & Sustainable Development

University / Organisation Name:

Zoological Survey of India (ZSI), Kolkata, West Bengal


1. Fellowship Details
Sanction Date: 31-03-2016
Year 2015-2016
Fellowship Duration 3 Years
BTGs:

1. Environmental assessment & management

2. Conservation and sustainable use of biodiversity

Project Site/ State/ Districts/ Villages Covered:

Hemis NP, Jammu & Kashmir GHNP, Himachal Pradesh, Askot WLS, Uttarakhand, DDBR, Arunachal Pradesh Singalila NP, West Bengal,DDBR, Arunachal Pradesh, Hemis National Park, Jammu & Kashmir

No of HRAs: 3 Nos
No of HJRFs: 10 Nos


2. Financial Details
Total Institutional Fellowships: Rs. 2,39,22,360/-
1st Year Fellowship Grant: Rs.78,15,720/-
2nd Year Fellowship Grant: Rs.78,15,720/-
Expenditure (in Rs.) (upto 31.12.16) :


3. Fellowship Objectives and Deliverables(HRAs)
Position Study Area Proposed Objectives Deliverables Achievements (upto 31.12.16)
HRA001 Trans, North-West, Himalaya (J&K) Number of Long-Term Ecological/ Environmental Monitoring (LTEM) sites established/ investigated/ robust datasets generated and scientific evidences generated across key sectors

• Identification of sites for LTEM

• Mainstreaming of long-term monitoring and building scientific evidence base across key sectors achieved

• Initial gradsects (Permanent Monitoring plots along altitudinal gradient) established across Indian Himalayan Region: In Hemis NP, Jammu & Kashmir, Trans Himalaya, one gradient was explored starting from 3600m to 5200m.

• Along this gradient, 6 light trap stations were established.

In Great Himalayan NP, Himachal Pradesh, North-Western Himalaya, three gradsects were established from 1500m to 3200m and 9 light trap stations were established.

• In Askot Wildlife Sanctuary, Uttarakhand, Western Himalaya, one gradsect was established from 600m to 3400m and 6 light trap stations were explored.

• In Dihang-Dibang Biosphere Reserve, Arunachal Pradesh, Eastern Himalaya, two gradsects were explored covering 400m to 2400m and 16 light trap stations were established.

• Along these gradsects, Nested Quadrates were established (three NQs per light trap station) which will be sampled in next field session for vegetation, disturbance and other significant habitat variables. These altitudinal sites along with NQs will be our permanent/long-term monitoring sites to explore relationship between Lepidopteran diversity and climatic variables.

• Two species of threatened Parnassini, Apollo butterflies were recorded from three Protected Areas: Hemis, GHNP and Askot.

HRA002 Central and East Himalaya (Sikkim and Uttarakhand) Effectiveness of approaches developed for conservation of species/ habitats/ areas

• Conservation frameworks developed/ strengthened/ implemented

• Awareness on biodiversity conservation and sustainable use promoted

• Historical Collection Localities have been identified from secondary literature survey. Survey will be conducted next year.

• Survey conducted in Singalila NP, West Bengal

HRA003 Eastern Himalaya (AP) Molecular Phylogenetic Work through DNA Barcoding to resolve species complexes and responses to changing climate

•A barcode database generation for future molecular and phylogenetic researches on Himalayan Lepidoptera

• Leg samples of Lepidoptera specimens have been targeted for DNA sources. Achieved success of DNA isolation from the subsamples.

• Amplification of the target gene segment was achieved through LCO-HCO primer pair. The PCR profile has been standardized and practiced.

• DNA sequencing of the target segment through Sanger sequencing protocol. Already standardized protocols have been used.

• DNA sequences were annotated in Sequence scanner and MEGA softwares.

 

 

4. Fellowship Objectives and Deliverables(HJRFs)
Position Study Area Proposed Objectives Deliverables Achievements (upto 31.12.16)
H-JRF001 Hemis National Park(HNP), J&K

To investigate the differential processes influencing the distribution pattern of Lepidoptera assemblages (moths and butterflies of HNP)

 

Generation of robust datasets generated through ecological monitoring at habitat level

• Generation of scientific evidences in the form of reports on biodiversity of HNP

• Robust data sets

• Thesis on the subject area

• Preliminary survey for selecting sampling sites undertaken during September 2016. (Specimen collection details provided in the attached report)

• Around 60 literatures on butterflies of Ladakh have been collected. Compilation of specie distribution data is under process. Specimens were sorted according to families and stretching was done.

• Seven sampling sites have been chosen as permanent monitoring sites - Sumdo, Nemaling, Nagdang village, Nagdang Pho-I-II-III and Markha, from National Park. At every sampling site, at least 2-3 sampling points have been chosen for repetitive sampling based on habitat type, elevation, and habitat quality. Species abundance data were also collected.

• The research team also approached local villagers for questionnaire about different issues pertaining to Lepidoptera diversity and agriculture practice to make an affective conservation plan.

H-JRF002 Great Himalayan National Park (GHNP), Himachal Pradesh

To investigate the differential processes influencing the Lepidoptera diversity of GHNP

 

Generation of robust datasets generated through ecological monitoring at habitat level

Generation of scientific evidences

• Generation of scientific evidences in the form of reports on biodiversity of GHNP

• Robust data sets

• Thesis on the subject area

 

• Preliminary surveys for selecting sampling sites undertaken during August-September in Great Himalaya National Park and November-December, 2016 in Askot Wildlife Sanctuary and Great Himalaya National Park. (Specimen collection details provided in the attached report)

• Reference collection of Lepidoptera from Zoological Records from the year of 2008, 2011, 2014 and compilation of total reference from 2008-2014.

• Butterfly data entry in BOLD (Barcoding Life Data System) format (Uttarakhand- 250 specimens) from secondary literature.

• Genitalia dissection and identification of Moths (26 individual samples of family Noctuidae and 3 individual samples of family Arctiidae, 5 individual samples of Lymantriidae and 9 individual samples of family Crambidae). • Validation of 200 Species of moths done, which recorded from Indian Himalayan Region.

• Validation of 180 Notodontidae species found in India from literature for checklist preparation.

• Collection data entry in BOLD format, Stretching and tagging of 600 Lepidoptera specimens done which were collected from Great Himalaya National Park.

• Literature Collection of Great Himalaya National Park.

• 300 Lepidoptera specimens of Valley of Flower National park has been stretched.

• Great Himalaya National Park: Two reconnaissance surveys were done in August and December in which a total of four trek routes (sampling gradients) have been selected. About 650 specimens of Moth have been collected from GHNP and the adjacent areas. Live photograph of all moth specimen was taken in the field. All specimen were sorted and stretched. From the August tour, 11 species of butterflies have been recorded with photographic evidence. Among them, two very rare species of Subfamily Parnassinae (Parnassius hardwickii & Papilio machaon) have been found.

H-JRF003 Askot Wildlife Sanctuary(AWS), Uttarakhand

To investigate how diverse are the Lepidopteran assemblages in AWS

 

Generation of robust datasets generated through ecological monitoring at habitat level

• Generation of scientific evidences in the form of reports on biodiversity of AWS

• Robust data sets

• Thesis on the subject area

 

• Two reconnaissance surveys were done in Great Himalayan National Park and Askot Wildlife Sanctuary in the months of August-September and November-December to select sampling sites (Specimen collection details provided in the attached report).

• Reference collection of Lepidoptera from Zoological Records from the year of 2008, 2011, 2014 and compilation of total reference from 2008-2014.

• Butterfly data entry in BOLD (Barcoding Life Data System) format (Sikkim- 256 species) from secondary literature.

• Genitalia dissection and identification of Moths.

• Validation of 250 Species of moths done, which recorded from Indian Himalayan Region.

• 180 Species detail from literature entered for preparing Notodontidae checklist.

• Collection data entry in BOLD format, Stretching and tagging of 570 Lepidoptera specimens done which were collected from Great Himalaya National Park and Askot Wildlife Sanctuary.

• Streching of 200 Lepidoptera specimen collected from Singalila National Park and Neora Valley National Park.

• Literature Collection of Askot Wildlife Sanctuary.

• Askot Wildlife Sanctuary: Two reconnaissance surveys were done in September and November in which two trek routes (sampling gradients) have been selected. About 570 specimens of Moth have been collected from AWLS and the adjacent areas. Live photograph of all moth specimen was taken in the field. All specimens were sorted and stretched. From the August-September tour, 12 species of butterflies have been recorded with photographic evidence. One rare and important species of Subfamily Parnassinae (Parnassius epaphus) was also recorded from the region.

H-JRF004 Kanchendzonga Biosphere Reserve(KBR)

To investigate the differential processes influencing the Lepidoptera diversity of KBR

 

Generation of robust datasets generated through ecological monitoring at habitat level

• Generation of scientific evidences in the form of reports on biodiversity of HNP

• Robust data sets

• Thesis on the subject area

 

H-JRF005 Singalila National Park(SNP)

To investigate the differential processes influencing the Lepidoptera diversity of SNP

 

Generation of robust datasets generated through ecological monitoring at habitat level

• Generation of scientific evidences in the form of reports on biodiversity of HNP

• Robust data sets

• Thesis on the subject area

 

• Preliminary surveys for selecting sampling sites undertaken during August-September and November-December, 2016 (Specimen collection details provided in the attached report)

• Compilation of species data for butterflies of central Himalaya was made for 669 species from Darjeeling district, WB and 319 species from Sikkim based on literature from State Fauna Series, ZSI.

• Literature on moths of central Himalayan region has been compiled for present status of work on moths in the region. 912 species of moth has been validated for species name using FUNET and LEPINDEX and the work is under progress.

SNP: Reconnaissance survey conducted in August-September 2016 for selecting sampling sites and collection of ecological parameter at habitat level. 137 samples were collected, as a team effort, in the first survey from outside the boundary of the National Park. Photographic sampling for 500 moths were done for the core and buffer zone. Repeat sampling conducted in in November- December 2016. 121 moths and 6 butterflies collected, as a team effort, in the repeat survey from outside the boundary of the National Park. Samples were sorted, stretched and stored in insect boxes for further taxonomic studies.

• NVP: Reconnaissance survey conducted in upper and lower NVP respectively in August-September and November-December, 2016 for selecting sampling sites and collection of ecological parameter at habitat level. 4197 samples of lepidopterans were collected, as a team effort, from the survey. 1197 samples has been sorted, stretched and stored in insect boxes for further taxonomic studies. Of these 121 samples have been processed for DNA analysis. 3000 samples have been sorted and is under processing for stretching and preservation.

H-JRF006 Dihang-Dibang Biosphere Reserve(DDBR)

To investigate the differential processes influencing the distribution pattern of Lepidoptera assemblages (moths and butterflies)

 

Generation of robust datasets generated through ecological monitoring at habitat level

• Generation of scientific evidences in the form of reports on biodiversity of HNP

• Robust data sets

• Thesis on the subject area

• Preliminary surveys for selecting sampling sites undertaken during August-September and November-December, 2016 (Specimen collection details provided in the attached report)

• We obtained 485 Lepidoptera specimens, over the two field (August- September 2016 and November to December 2016) to assess the species and taxonomic diversity of Dihang Dibang Biosphere reserve and targeted four different route to assess environmental factors and species diversity. Those routes are having the highest altitudinal gradients up to 4000 m in Eastern Himalaya. All collected specimens have been stretched and documented. Photographic documentation is in process. The processed data has been gathered in Excel Workbook (BOLD format) and genitalia base identification is in process.

H-JRF007 Dihang-Dibang Biosphere Reserve(DDBR) To investigate the differential processes influencing the distribution pattern of Lepidoptera assemblages (moths and butterflies) in DDBR

Generation of robust datasets generated through ecological monitoring at habitat level

• Generation of scientific evidences in the form of reports on biodiversity of HNP

• Robust data sets

• Thesis on the subject area

 

• Preliminary surveys for selecting sampling sites undertaken during August-September in SNP and November-December, 2016 in DDBR (Specimen collection details provided in the attached report)

• About 175 Literature of butterflies of Himalayan region were collected, including 21 records of ZSI. Compilation of specie data is now going on (about 41 papers data has complied). Compilation of state fauna series, ZSI is in progress.

• SNP: A total about 450 specimen of moths have been collected from SNP and their adjacent areas. Live photographs of moths were taken in field. All specimens were sorted and stretched. From this tour, we have recorded 18 species of butterflies with photographic evidence. Among them, mostly dominated genera Lethe with 6 species, of which Lethe atkinsonia, Lethe maitrya and Lethe goalpara were recorded. These species are rare and restricted to elevation above 1800m, as per Evans, 1932.

• DDBR- From this tour about 200 specimens of moths were collected and all were sorted and stretched. About 139 species of butterflies were recorded in 17 transects from various sites. Among these butterflies most interesting finding is Drupadia scaeva cyara. The species is first time record from Arunachal Pradesh, eastern Himalaya, and recorded after 83 years from India (last recorded by Eliot, 1934 from Gangtok). This is also first photographic evidence from mainland India. Other interesting findings include Elymnias patna, Hypolimnas misippus, Tajuria sp etc.

H-JRF008 Eastern Himalaya (Arunachal Pradesh) Study of molecular taxonomy in AP

• Report on status of molecular taxonomy of AP

 

• Sub-samples of legs from the Lepidoptera specimens from all the five study sites were collected with proper labeling for downstream work with DNA.

• DNA isolation protocol was standardized using phenol-choloform method.

• PCR protocol was standardized using LCO-HCO primer pair for amplification of the target gene segment.

• DNA sequencing of the target gene segment was accomplished. •

H-JRF009 Eastern Himalaya (Meghalaya) Morphology-based taxonomy

• Report on status of molecular taxonomy of AP

 

• Field work conducted in GHNP, Western Himalaya during August-September, 2016. In this field survey, a total of 403 lepidopteran and 73 other insect specimens were collected from a single elevational gradient ranging from 1500 m to 3600 m. Details of collections were provided in the attached document.

• For identification, stretched lepidopteran specimens from five biogeographic provinces viz. Trans, North West, West, Central and Eastern Himalayas were sorted according to families. From preliminary observation, specimens belonging to 25 lepidopteran families were found. These are Papillionidae, Nymphalidae, Lycaenidae, Pieridae, Hesperiidae, Totricidae, Pterophoridae, Cossidae, Zygaenidae, Callidulidae, Limacodidae, Pyralidae, Crambidae, Lasiocampidae, Saturniidae, Sphingidae, Eupterotidae, Drepanidae, Uraniidae, Geometridae, Notodontidae, Erebidae, Euteliidae, Nolidae, Noctuidae. Among these, Geometridae comprises major portion of the collection. Other notable families include Erebidae, Noctuidae, Crambidae and Sphingidae. Morpho species wise specimens of each family have been sorted.

• Genitalia dissection of specimens belonging to Geometridae, Eupterotidae, Sphingidae and Noctuidae are in progress.

H-JRF0010 Western, Central and Eastern Himalaya

Modelling on GIS-framework and data compilation

comparison of robust datasets generated through ecological monitoring

• Robust database on species/ habitats

• Strategy for conservation

 

• Toposheets for study sites acquired from Survey of India.

• Initial boundary was demarcated for all PAs along with initial sampling routes and sampling points were marked at every 200 m vertical distance & vegetation type.

• Satellite data for DEM (ASTER G- DEM data) & NDVI (MODIS) data downloaded from United States Geological Survey (USGS), climatic variable data downloading are in progress from WorldClim for different temporal scale.

 

 

 

S.No. Name Date of Joining Name of the PI Qualification Research Title
1. Dr. Abesh Kumar Sanyal 03.06.2016 Dr. Kailash Chandra Ph.D. Lepidoptera (Insecta) as a Potential Indicator Taxa for Tracking Climate Change in the Indian Himalayan Landscape
2. Dr. Arockia Lenin 13.10.2016 Dr. Kailash Chandra Ph.D. Lepidoptera (Insecta) as a Potential Indicator Taxa for Tracking Climate Change in the Indian Himalayan Landscape
3. Dr. John Caleb 01-03-2017 Dr. Kailash Chandra Ph.D. Lepidoptera (Insecta) as a Potential Indicator Taxa for Tracking Climate Change in the Indian Himalayan Landscape
S.No. Name Date of Joining Name of the PI Qualification Research Title
1. Mohd. Ali 29.09.2016 Dr. Kailash Chandra M.Sc. Assessing Diversity & Distribution of Lepidoptera focusing on Apollos (Papilionidae: Parnassini) as Flagship for Biodiversity Conservation in Trans Himalayan Landscape of Ladakh.
2. Kaushik Mallick 06.05.2016 Dr. Kailash Chandra M.Sc. Diversity and Distributional Pattern of Family Geometridae in Great Himalayan National Park, Himachal Pradesh
3. Uttaran Bandyopadhyay 14.05.2016 Dr. Kailash Chandra M.Sc. Diversity and distribution pattern of Moths (Lepidoptera: Heterocera) in special emphasis on family Noctuidae in Askot WLS, Uttarakhand.
4. Rahul Ranjan 24-02-2017 Dr. Kailash Chandra M.Sc. Lepidoptera (Insecta)as potential Indicator- Taxa for tracking Climate Change in the Indian Himalayan Landscape
5. Kamalika Bhattacharyya 14.05.2016 Dr. Kailash Chandra M.Sc. Moth (Lepidoptera) assemblages along altitudinal gradient in Singalila and Neora Valley National Park, Central Himalaya, India-An ecological and molecular approach emphasising on Noctuidae
6. Subrata Gayen 14.05.2016 Dr. Kailash Chandra M.Sc. Lepidoptera (Insecta) as a Potential Indicator Taxa for Tracking Climate Change in the Indian Himalayan Landscape
7. Gaurab Nandi Das 14.05.2016 Dr. Kailash Chandra M.Sc. Lepidoptera (Insecta) as a Potential Indicator Taxa for Tracking Climate Change in the Indian Himalayan Landscape
8. Sumantika Chatterjee 03.05.2016 Dr. Kailash Chandra M.Sc. Lepidoptera (Insecta) as potential indicator-Taxa for tracking Climate Change in the Indian Himalayan Landscape
9. Angshuman Raha 03.05.2016 Dr. Kailash Chandra M.Sc. Lepidoptera (Insecta) as a Potential Indicator Taxa for Tracking Climate Change in the Indian Himalayan Landscape
10. Rohit Kumar Jaiswal 15.05.2016 Dr. Kailash Chandra M.Sc. Lepidoptera (Insecta) as a Potential Indicator Taxa for Tracking Climate Change in the Indian Himalayan Landscape